The model of cerebral angiospasm using human blood at rats

Objective. To create a model of cerebral angiospasm (CA) due to non-traumatic subarachnoid hemorrhage (SAH) at laboratory white rat using human blood and to study the effects of forteplase (recombinant staphylokinase fibrinolytic) Materials and methods. The study was performed on 13 laboratory white rat weighing 180-220 g. Autologous venous and human venous blood was injected in cistern magna twice. The neurological assessment of the animals was performed daily. The rat brain for morphological study was taken at 5th day. The microscopy assessment was done at the level of the upper third of the basilar artery using special histochemical stains (by Gram-Weigert, by Lie). The slide description was performed using light microscope Leica DM 1000 with an increase of 400-1000 times, the photomicrography was performed using digital camera Leica EC 3. The intravenous injection of forteplase was used for studying effects on rat fibrinolytic system Results. The direct qualitative features of cerebral vasospasm (smooth muscle cells (SMC) proliferation, SMC cytoplasm vacuolization, internal elastic membrane wrinkling, hypercontractive changes of smooth muscle cells, capillary blood flow violations in the level of the slides) were identified in all animals without any difference between autologous venous and human venous blood groups. There was no evidence of forteplase effect on rat fibrinolytic system. Conclusion. The proposed model of human venous blood injection in cisterna magna of the rat demonstrates CF due to non-traumatic SAH. Rat fibrinolytic system is insusceptible to forteplase.

нетравматическое субарахноидальное кровоизлияние, сосудистый спазм, экспериментальная биологическая модель, белая крыса, фибринолиз, Nontraumatic subarachnoid hemorrhage, cerebral vasospasm, in vivo rat model, fibrinolysis

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