The modelling of cerebral angiospasm because of non-traumatic subarachnoid hemorrhage using rat model

Objective. To study the morphological changes in basilar artery and brain tissues during experimental nontraumatic subarachnoid haemorrhage using laboratory white rat as well as to compare the spasmogenic effect of venous and arterial autologous blood. Materials and methods. The study was performed using 18 laboratory white rat weighing 180-220 g. Venous and arterial autologous blood was injected in cistern magna twice. Neurological assessment of the animals was performed daily. Brain for morphological study was taken out at the 3rd, 4th, 5th, 6th and 7th day. Microscopy assessment was performed at the level of the upper third of the basilar artery using special histochemical stains (by Gram-Weigert, by Lie). Slide description was performed at the light microscope Leica DM 1000 with an increase of 400-1000 times, photomicrography was performed using digital camera Leica EC 3. Results. The direct qualitative evidences of cerebral vasospasm (reduction in luminal diameter of the basilar artery, thickening of the vessel wall, internal elastic membrane wrinkling, hypercontractive changes of smooth muscle cells) were identified. Vasospasm was detected on the 4th day, with maximum expression at day 5 and regression by 6-7 day. The direct indicators of brain ishemia in the form of capillary blood flow violations were identified at the pons level. There was no difference in spasmogenic effect comparing venous and arterial blood. Conclusion. The suggested model of autologous blood injection in cisterna magna of the rat is adequate and appropriate for further study of the cerebral vasospasm after nontraumatic subarachnoid haemorrhage as well as for investigation of the methods for cerebral vasospasm prevention and treatment.

нетравматическое субарахноидальное кровоизлияние, сосудистый спазм, экспериментальная биологическая модель, белая крыса, nontraumatic subarachnoid haemorrhage, cerebral vasospasm, in vivo rat model

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